How does sample type affect which type of Freeze Dry Machine is ideal? In order to properly freeze dry samples, a differential of 15- 20°C between the eutectic temperature (freezing point) of the sample and the lyophilizer is needed. Most biological samples are sufficiently frozen by a standard system reaching -50°C. For samples such as HPLC preparations in acetonitrile, which has a eutectic point of approximately -42°C, a much lower temperature will need to be achieved. A cascade-type collector which can reach down to -84°C will be able to accommodate this. It should be noted that with many lab professionals switching to methanol for these processes due to its availability and affordability, an ultra-low temperature lyophilizer, able to hit as low as -105°C may be required due to methanol having a eutectic point of -97.6°C.
4 Signs That You Should Service or Replace Your Freeze Dryer
1. Your application has changed. The freeze dryer was intended for aqueous samples, now solvents or acids are used. A temperature differential is required for proper freeze drying; an upgrade to a cascade model is recommended.
2. The freeze dryer was inherited from another lab. A used freeze dryer can cause problems. Rubber parts dry out and crack, decontamination may not have been done properly. A used pump can have corrosion that can’t be seen until it’s too late.
3. Sharing a community freeze dryer. Personal freeze dryers used by a single researcher are more popular than ever. No more guessing if an oil change was done, or if a harmful sample is being processed.
4. The unit no longer pulls a good vacuum. Gaskets, hoses and all rubber components break down over time causing poor vacuum and refrigeration leaks. More information about Freeze Dry Machine please click https://www.gf-machine.com/